The artist has donated the full amount of his £25,000 award prize money to the trust for venues, which works to save the UK's independent live music spots.
He was awarded the cheque on October 16 as his new release the album was named the best record of the previous year.
Fender has chosen to donate the funds to the trust, in acknowledgment of the essential function small stages fulfilled in his beginning days.
"I personally wouldn't be doing this success if it weren't for all the shows I played around the region, and beyond, when I was beginning," remarked Sam Fender. "These locations are iconic, but they are struggling."
From early 2023 onward, over 150 of these venues have permanently closed – about 16 percent of the entire UK sector.
In the last year, major artists such as Pulp, the group Coldplay, the singer Katy Perry and Ed Sheeran have all backed the Music Venues Trust in its attempts to help venues survive, by adding a small levy to their ticket costs, which benefits local stages.
Sam Fender also participated, raising more than £100,000 on his 2024 arena tour to assist 38 local spots across Britain.
The donation helped locations that were dealing with possible closure due to difficulties arising from water damage, fire incidents and bereavements, as well as regulatory hurdles, court cases and noise complaints.
Some locations received financial assistance in upgrading amenities and technical equipment that positively impacted musicians and fans.
"The idea that funds from performances in major spaces backs the independent spots, where it kicks off for performers like me, is just logical," Sam Fender has commented.
Fender is not the first artist to donate his Mercury Prize prize money to charitable organizations.
When Pulp received the trophy in the mid-90s for their album Different Class, frontman the singer declared that the group would donate their winnings to the organization the charity.
In 2002, musician Ms Dynamite divided her award between a few good causes, such as the NSPCC and a charity for Sickle Cell.
"I also contributed a thousand pounds to the center, to their gymnastics class, because I practiced gymnastics when I was a child and they needed new equipment," Ms Dynamite told outlets.
In recent years, they gave their prize money to the youth organization that supported their band, as well as other independent music bodies.
Additionally the 1994 recipients they contributed their award to a charity for MS after a friend was identified with the condition.
"Receiving the award was ample," remarked artist Small. "The prize was the bonus but we didn't need the extra, because we had the main prize. So our success impacted another person's life."
In the past week, the Music Venues Trust announced it had rescued a couple of grassroots venues in south-east England, by placing them under public stewardship.
One venue in the city and the other in the city were purchased under the community ownership scheme, which is supported by the arts council and enthusiasts who buy "stakes" in the properties.